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Gene Cloning Techniques
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Gene Cloning Techniques
Cytotoxicity
Chronic Toxicology
Genotoxicity
Preventive assay
Histopathology
Enzymic assay
Brine Shrimp (Artemia) Lethality Bioassay
Lethality bioassay with Fluorescence staining
Assessment of Organic Pollutants
In vitro Assessments of Organic dyes
Fabrication of Nano-biomaterials
Nano-drug delivery studies
Nano-Toxicological studies
In vitro biodegradation studies
Analysis of Tissue Engineering biomaterials
Characterizations of Nanomaterials
XRD
FE-SEM-EDX
Molecular Docking
Molecular Simulation
QSAR Studies
Phylogenotic Analysis
Sequence Analysis
Isolation
In vitro Anti-bacterial and Anti-fungal assays
MIC and MBC methods
Well-diffusion method and Disc Diffusion Method
Colony forming unit (CFU) and Agar Bioassay
Microbial Morphological Observations
Live/Dead Fluorescence staining
Anti-biofilm assay
AO/EB and Crystal Violet staining
Specific Protein Isolation
Antimicrobial Peptide Isolation (AMPs)
Microbial Protein Expression
Gene Cloning Techniques
RNA isolation and qRT-PCR analysis
Quantification of Gene and Cytokines expressions
+ SDS-PAGE Analysis
In vitro Polarization studies
Next-Generation Sequencing Analysis
MTT
CCK-8
LDH
Salphorhodamine-B
Live/Dead staining (Eg., AO/EB and FDA/PI)
Cell Nuclear staining (Eg., DAPI and Hoechst)
ROS Generation Assay (E.g., DCFH-DA)
Mitochondrial membrane potential (MMP)
COMET (DNA Fragmentation) Assay (Single Cell Gel Electrophoresis)
Immunofluorescence staining (Different anti-bodies)
Cell apoptosis (Annexin V-FITC/PI) analysis by Flow cytometry
Cell cycle analysis (Pl RNase stain) by Flow cytometry
Cell morphological analysis - Phase Contrast staining methods
Tryphan blue exclusion assay
Giemsa s staining
Alizerin Red staining
Red oil O staining
Crystal violet
Cell compatibility analysis
Cell migration assay (Wound scratch model)
Cell proliferation assay
Cellular uptake analysis
Alkaline Phosphatase (ALP) Assay
RAW264.7 murine macrophages-induced Inflammatory assay
Albumin denaturation assay
HRBC membrane stabilization method
Lipoxygenase inhibition assays
Radical scavenging (DPPH)assay
Alpha amylase inhibition assay
Alpha glucosidase inhibition assay
Glucose consumption test
Glucose uptake (2-NBDG)
FTIR
UV-visible
XPS
DLS
TGA-DSC
Raman spectroscopy
HR-TEM
AFM microscopy
Culture and Identification of Microbial Strains
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